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Improving the crystallization and melting characteristics of cocoa butter substitute by blending with krabok seed fat.

ABSTRACT: This work investigated the crystallization and melting behavior of a commercial cocoa butter substitute (CBS) blended with 10-80% (by weight) of a hard lauric fat called krabok seed fat (KSF). The aim was to find CBS-KSF blends with improved crystallization and melting characteristics from that of the CBS. It was found that the addition of 10-80% KSF to CBS improved the melting properties of the CBS. However, 10-20% KSF resulted in too high solid fat content (SFC) values at the body temperature (37 °C) which would lead to waxy mouth feel. Adding 30-40% KSF resulted in better melting profiles than 10-20% KSF with SFC values < 3% at 37 °C and SFC curves most similar to cocoa butter. However, 40% KSF led to a significant decrease in the crystallization rate from that of CBS and a significant increase in the average crystal size. With 60-80% KSF, although the blends melted completely at the body temperature, their crystallization rates were significantly reduced. All CBS-KSF blends crystallized into β' structure. Therefore, the addition of 30% KSF to the CBS is recommended for industrial use to obtain compound chocolate with improved quality.

Supplementary information

The online version contains supplementary material available at 10.1007/s13197-022-05513-1.

SUBMITTER: Podchong P

PROVIDER: S-EPMC9525477 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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Improving the crystallization and melting characteristics of cocoa butter substitute by blending with krabok seed fat.

Podchong Pawitchaya P Inbumrung Patraporn P Klinkesorn Utai U Sonwai Sopark S

Journal of food science and technology 20220706 11

This work investigated the crystallization and melting behavior of a commercial cocoa butter substitute (CBS) blended with 10-80% (by weight) of a hard lauric fat called krabok seed fat (KSF). The aim was to find CBS-KSF blends with improved crystallization and melting characteristics from that of the CBS. It was found that the addition of 10-80% KSF to CBS improved the melting properties of the CBS. However, 10-20% KSF resulted in too high solid fat content (SFC) values at the body temperature ...[more]

PMID: 36193485

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Project description:BACKGROUND:Cocoa butter (CB) extracted from cocoa beans (Theobroma cacao) is the main raw material for chocolate production, but CB supply is insufficient due to the increased chocolate demand and limited CB production. CB is mainly composed of three different kinds of triacylglycerols (TAGs), 1,3-dipalmitoyl-2-oleoyl-glycerol (POP, C16:0-C18:1-C16:0), 1-palmitoyl-3-stearoyl-2-oleoyl-glycerol (POS, C16:0-C18:1-C18:0) and 1,3-distearoyl-2-oleoyl-glycerol (SOS, C18:0-C18:1-C18:0). In general, Saccharomyces cerevisiae produces TAGs as storage lipids, which consist of C16 and C18 fatty acids. However, cocoa butter-like lipids (CBL, which are composed of POP, POS and SOS) are not among the major TAG forms in yeast. TAG biosynthesis is mainly catalyzed by three enzymes: glycerol-3-phosphate acyltransferase (GPAT), lysophospholipid acyltransferase (LPAT) and diacylglycerol acyltransferase (DGAT), and it is essential to modulate the yeast TAG biosynthetic pathway for higher CBL production. RESULTS:We cloned seven GPAT genes and three LPAT genes from cocoa cDNA, in order to screen for CBL biosynthetic gene candidates. By expressing these cloned cocoa genes and two synthesized cocoa DGAT genes in S. cerevisiae, we successfully increased total fatty acid production, TAG production and CBL production in some of the strains. In the best producer, the potential CBL content was eightfold higher than the control strain, suggesting the cocoa genes expressed in this strain were functional and might be responsible for CBL biosynthesis. Moreover, the potential CBL content increased 134-fold over the control Y29-TcD1 (IMX581 sct1Δ ale1Δ lro1Δ dga1Δ with TcDGAT1 expression) in strain Y29-441 (IMX581 sct1Δ ale1Δ lro1Δ dga1Δ with TcGPAT4, TcLPAT4 and TcDGAT1 expression) further suggesting cocoa GPAT and LPAT genes functioned in yeast. CONCLUSIONS:We demonstrated that cocoa TAG biosynthetic genes functioned in S. cerevisiae and identified cocoa genes that may be involved in CBL production. Moreover, we found that expression of some cocoa CBL biosynthetic genes improved potential CBL production in S. cerevisiae, showing that metabolic engineering of yeast for cocoa butter production can be realized by manipulating the key enzymes GPAT, LPAT and DGAT in the TAG biosynthetic pathway.

Dataset Information

Improving the crystallization and melting characteristics of cocoa butter substitute by blending with krabok seed fat.

Supplementary information

Publications

Improving the crystallization and melting characteristics of cocoa butter substitute by blending with krabok seed fat.

Similar Datasets

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