An expedient ion chromatography based method for high-throughput analysis of phytic acid in groundnut kernels.

ABSTRACT: A study was made to expedite ion chromatography method using IonPac analytical column and self-regenerating anion suppressor for phytic acid determination in groundnut seeds and compared with a widely adopted spectrophotometric method based on enzymatic hydrolysis. The Ion Chromatography method equipped with AG11 guard and AS11 analytical columns in isocratic mode using 65 mM NaOH mobile phase at 1 mL min^-1 flow rate showed a sharp peak for phytic acid with a retention time of 2.42 ± 0.2 min. The peak area was plotted v/s concentration showed linearity with an R² value of 0.997, detection limit of 0.028 mg L^-1 and recovery of 98% as against R² value of 0.988 and detection limit of 0.065 mg L^-1 in the spectrophotometric method. The stud

SUBMITTER: Verma A 

PROVIDER: S-EPMC9525535 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature