Development of a duplex qPCR assay with locked nucleic acid probes for A, B and E kappa-casein variants detection.

ABSTRACT: Milk proteins determine important milk technological characteristics. Among caseins, Ƙ-casein has been correlated with fat and protein content and cheese yield. Fourteen Ƙ-caseins variants have been described but the alleles A, B and E are the most important ones due to their frequency and/or influence on the technological aptitudes of milk. Therefore, in the present study two different duplex qPCR assays with locked nucleic acid probes (for positions 13104 and 13124 of the Ƙ-casein gene) were developed for the detection of A, B and E variants. Firstly, DNA isolation method from milk somatic cells and hair was optimised. The developed 13124-qPCR assay showed an increased sensitivity reaching up to 6.7 copies DNA copies/reaction at a 95% confidence level with A, B and E alleles reference sa

SUBMITTER: Jimenez-Montenegro L 

PROVIDER: S-EPMC9525573 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature