SiRNA-mediated gene knockdown via electroporation in hydrozoan jellyfish embryos.

ABSTRACT: As the sister group to bilaterians, cnidarians stand in a unique phylogenetic position that provides insight into evolutionary aspects of animal development, physiology, and behavior. While cnidarians are classified into two types, sessile polyps and free-swimming medusae, most studies at the cellular and molecular levels have been conducted on representative polyp-type cnidarians and have focused on establishing techniques of genetic manipulation. Recently, gene knockdown by delivery of short hairpin RNAs into eggs via electroporation has been introduced in two polyp-type cnidarians, Nematostella vectensis and Hydractinia symbiolongicarpus, enabling systematic loss-of-function experiments. By contrast, current methods of genetic manipulation for most medusa-type cnidarians, or jellyfish,

SUBMITTER: Masuda-Ozawa T 

PROVIDER: S-EPMC9525680 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature