Connexin 37 Regulates the Kv1.3 Pathway and Promotes the Development of Atherosclerosis.

ABSTRACT:

Objective

To investigate the mechanism of Connexin 37 (Cx37) and Kv1.3 pathways in atherosclerosis (AS).

Methods

ApoE^-/- mice were given a high-fat diet to establish atherosclerosis (AS) model, and macrophages in mice were isolated and extracted to transfect Cx37 vectors with silencing or overexpressing, and Kv1.3 pathway blockers were used to inhibit the pathway activity. The indexes of body weight, blood glucose, and blood lipid of mice were collected. The protein and mRNA expression levels of Cx37 and Kv1.3 were detected by reverse transcription-PCR (RT-PCR), Western blot, and immunofluorescence technique. Oil red O staining was used to observe plaque area. Masson staining was used to detect collagen content. The concentrations of chemokine CCL7 were quantified

SUBMITTER: Liao M 

PROVIDER: S-EPMC9525889 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature