Roseburia intestinalis stimulates TLR5-dependent intestinal immunity against Crohn's disease.

ABSTRACT:

Background

Crohn's disease (CD) is a chronic inflammatory disorder characterized by intestinal immune dysfunction. Multiple factors, including gut dysbiosis, are involved in the pathogenesis of CD. However, the effect of commensal bacteria on controlling the inflammatory response in individuals with CD remains unclear.

Methods

We detected Toll-like receptor 2 (TLR2), TLR4, and TLR5 expression in Roseburia intestinalis (R. intestinalis)-treated mice with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis. Then, we quantified the signs of colonic inflammation, the proportion of regulatory T cells (Tregs) and the expression of thymic stromal lymphopoietin (TSLP) and transforming growth factor (TGF)-β in TLR-5-deficient (Tlr5^-/-) mice, bone marrow chimera mice

SUBMITTER: Shen Z 

PROVIDER: S-EPMC9526137 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature