Overexpression of CYP11A1 recovers cell cycle distribution in renal cell carcinoma Caki-1.

ABSTRACT:

Background

Clear cell renal carcinoma is commonly known for its metastasis propensity to outspread to other organs and is asymptomatic in the early stage. Recent studies have shown that deficiencies in CYP11A1 expression can lead to fatal adrenal failure if left untreated and are associated with downstream regulation in various cancer types. However, the molecular mechanisms of CYP11A1 and kidney cancer proliferation remain unclear.

Methods

Normal and renal carcinoma cell lines (HEK293 and Caki-1) were transfected with plasmid encoding CYP11A1 to overexpress the P450scc protein. Cell cycle distribution was investigated using flow cytometry. The expression of proteins related to C-Raf/ERK/JNK/p38 signaling pathways was examined using western blot.

Results

We observed t

SUBMITTER: Ong HTM 

PROVIDER: S-EPMC9526279 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature