Assigning Culicoides larvae to species using DNA barcoding of adult females and phylogenetic associations.

ABSTRACT:

Background

Orbivirus-induced hemorrhagic diseases cause high mortality in wild and captive white-tailed deer in North America. The role of different Culicoides species in Orbivirus transmission outside of areas of intensive animal production has not been established. At our study location, bluetongue virus (BTV) RNA-positive female Culicoides debilipalpis pools have been detected annually since 2012 when BTV transmission was noted in a captive deer herd. Identifying specific larval habitats of suspected vectors at active transmission sites is crucial both for identifying the source of the vectors and for subsequently planning intervention actions. Since C. debilipalpis larvae are known to develop in tree holes, this study was designed to use DNA barcoding to identify larvae collect

SUBMITTER: Jin T 

PROVIDER: S-EPMC9526334 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature