A novel alkane monooxygenase (alkB) clade revealed by massive genomic survey and its dissemination association with IS elements.

ABSTRACT:

Background

Alkanes are important components of fossil energy, such as crude oil. The alkane monooxygenase encoded by alkB gene performs the initial step of alkane degradation under aerobic conditions. The alkB gene is well studied due to its ubiquity as well as the availability of experimentally functional evidence. The alkBFGHJKL and alkST clusters are special kind of alkB-type alkane hydroxylase system, which encode all proteins necessary for converting alkanes into corresponding fatty acids.

Methods

To explore whether the alkBFGHJKL and alkST clusters were widely distributed, we performed a large-scale analysis of isolate and metagenome assembled genome data (>390,000 genomes) to identify these clusters, together with distr

SUBMITTER: Wang S 

PROVIDER: S-EPMC9526415 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature