Inhibition of CCR8 attenuates Ang Ⅱ-induced vascular smooth muscle cell injury by suppressing the MAPK/NF-κB pathway.

ABSTRACT:

Objectives

Hyperinsulinemia, secondary to insulin resistance, may lead to vascular smooth muscle cell dysfunction. In the present research, we aimed to investigate the effect of Chemokine receptor 8 (CCR8) on angiotensin II (Ang II)-induced dysfunction of vascular smooth muscle cells (VSMCs) and to explore the underlying molecular mechanism.

Materials and methods

The expression of CCR8 was analyzed in diabetics and normal people by RT-PCR and ELISA. CCK-8 assay and transwell were used to explore cell proliferation and migration, and ELISA was used to measure the content of IL-6 and TNF-α. Reactive oxygen species (ROS) kit was employed to measure ROS generation.

Results

The results revealed that CCR8 was highly expressed in diabetics and Ang Ⅱ-induced VSMCs. Further st

SUBMITTER: Liao L 

PROVIDER: S-EPMC9526881 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature