Combination of long-term ¹³CO₂ labeling and isotopolog profiling allows turnover analysis of photosynthetic pigments in Arabidopsis leaves.

ABSTRACT:

Background

Living cells maintain and adjust structural and functional integrity by continual synthesis and degradation of metabolites and macromolecules. The maintenance and adjustment of thylakoid membrane involve turnover of photosynthetic pigments along with subunits of protein complexes. Quantifying their turnover is essential to understand the mechanisms of homeostasis and long-term acclimation of photosynthetic apparatus. Here we report methods combining whole-plant long-term ¹³CO₂ labeling and liquid chromatography - mass spectrometry (LC-MS) analysis to determine the size of non-labeled population (NLP) of carotenoids and chlorophylls (Chl) in leaf pigment extracts of partially ¹³C-labeled plants.

Results

The labeling chamber enabled

SUBMITTER: Banh AT 

PROVIDER: S-EPMC9526918 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature