MiR21 deletion in osteocytes has direct and indirect effects on skeletal muscle in a sex-dimorphic manner in mice.

ABSTRACT:

Background

Osteocytic microRNA21 (miR21) removal alters cytokine production and bone mass by modulating osteoclast and osteoblast differentiation and activity. Removing osteocytic miR21 increases osteoclast/osteoblast numbers and bone mass in male mice, whereas it decreases osteoclasts/osteoblasts without affecting bone mass in female mice. On the other hand, it leads to sex-independent increases in bone mechanical properties. Because changes in bone remodeling and strength affect skeletal muscle through bone-muscle crosstalk, we investigated whether osteocytic miR21 deletion influences skeletal muscle.

Methods

miR21fl/fl mice and 8kbDMP1-Cre mice were mated to obtain miR21-deficient mice primarily in the osteocyte (OtmiR21Δ) and littermate controls (miR21fl/fl). Four-month-

SUBMITTER: Essex AL 

PROVIDER: S-EPMC9526971 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature