Analysis of genetic diversity among Chinese Cyclocybe chaxingu strains using ISSR and SRAP markers.

ABSTRACT:

Background

Cyclocybe chaxingu is an edible and medicinal fungal species commonly cultivated in China. The major problems currently facing by growers of C. chaxingu is the random labeling of strains and strains aging and degeneration. Therefore, an evaluation of genetic diversity is essential for the conservation and reproducing programs of this species.

Methods

In the present study, 24 widely cultivated strains were collected from the main producing areas of China, and the genetic diversity analysis was performed. DNA polymorphism among these Chinese C. chaxingu strains was analyzed using inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers.

Results

Eight ISSR primers amplified a total of 75 DNA fragments

SUBMITTER: Liu JH 

PROVIDER: S-EPMC9527026 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature