Enterobacteria Phage Ac3's Genome Annotation and Host Range Analysis Against the ECOR Reference Library.

ABSTRACT: Host range analyses and genome sequencing/annotation of bacteriophage isolates allow more effective development of tools for applications in medicine, agriculture, and the environment and expand our understanding of phage biology. Here we present the complete sequence of phage Ac3's assembled and annotated genome (accession OK040907). Originally referred to simply as "3," Ac3 has previously been described as a T4-like bacteriophage belonging to the Myoviridae family in the Caudovirales order of tailed bacteriophages. Using a combination of spot tests and full plate plaque assays, Ac3's permissive and adsorptive host range were evaluated against the ECOR Reference Library; a panel of 72 Escherichia coli isolates meant to represent the diversity of E. coli. Spot a

SUBMITTER: Farquharson EL 

PROVIDER: S-EPMC9527048 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature