Production and Characterization of Rat Monoclonal Antibodies against the PAL Antigen of Legionella spp.

ABSTRACT: The purpose of this work was to obtain genus-specific monoclonal antibodies against the Legionella spp. recombinant PAL protein, which will subsequently allow to use them as a basis for the development of new express tests for pathogenic legionella detection. A short three-week immunization protocol for Wistar rats was used to generate rat-mouse heterohybridomas producing antibodies against PAL. Mouse myeloma cell line Sp2/0-Ag14 served as the fusion partner. Hybridization was performed using two methods: PEG-mediated fusion and electrofusion. Subsequent screening was performed by indirect solid-phase ELISA against the target protein rPAL. Specificity analysis was performed by dot-blot using a panel of lysates obtained from 39 pure cultures of different strains, which included close

SUBMITTER: Zeninskaya NA 

PROVIDER: S-EPMC9527138 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature