Crystallographic and X-ray scattering study of RdfS, a recombination directionality factor from an integrative and conjugative element.

ABSTRACT: The recombination directionality factors from Mesorhizobium spp. (RdfS) are involved in regulating the excision and transfer of integrative and conjugative elements. Here, solution small-angle X-ray scattering, and crystallization and preliminary structure solution of RdfS from Mesorhizobium japonicum R7A are presented. RdfS crystallizes in space group P2₁2₁2₁, with evidence of eightfold rotational crystallographic/noncrystallographic symmetry. Initial structure determination by molecular replacement using ab initio models yielded a partial model (three molecules), which was completed after manual inspection revealed unmodelled electron density. The finalized crystal structure of RdfS reveals a head-to-tail polymer forming left-handed superhelices with larg

SUBMITTER: Verdonk CJ 

PROVIDER: S-EPMC9527761 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature