A Preparative Mass Spectrometer to Deposit Intact Large Native Protein Complexes.

ABSTRACT: Electrospray ion-beam deposition (ES-IBD) is a versatile tool to study the structure and reactivity of molecules from small metal clusters to large protein assemblies. It brings molecules gently into the gas phase, where they can be accurately manipulated and purified, followed by controlled deposition onto various substrates. In combination with imaging techniques, direct structural information on well-defined molecules can be obtained, which is essential to test and interpret results from indirect mass spectrometry techniques. To date, ion-beam deposition experiments are limited to a small number of custom instruments worldwide, and there are no commercial alternatives. Here we present a module that adds ion-beam deposition capabilities to a popular commercial MS platform (Thermo Scienti

SUBMITTER: Fremdling P 

PROVIDER: S-EPMC9527803 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature