Engineering Yarrowia lipolytica for the sustainable production of β-farnesene from waste oil feedstock.

ABSTRACT:

Background

β-Farnesene is a sesquiterpene with versatile industrial applications. The production of β-farnesene from waste lipid feedstock is an attractive method for sustainable production and recycling waste oil. Yarrowia lipolytica is an unconventional oleaginous yeast, which can use lipid feedstock and has great potential to synthesize acetyl-CoA-derived chemicals.

Results

In this study, we engineered Y. lipolytica to produce β-farnesene from lipid feedstock. To direct the flux of acetyl-CoA, which is generated from lipid β-oxidation, to β-farnesene synthesis, the mevalonate synthesis pathway was compartmentalized into peroxisomes. β-Farnesene production was then engineered by the protein engineering of β-farnesene synthase and pathway engineering. The regulation of lipi

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9528160 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature