Carbamylation of Integrin α _IIb β ₃: The Mechanistic Link to Platelet Dysfunction in ESKD.

ABSTRACT:

Background

Bleeding diatheses, common among patients with ESKD, can lead to serious complications, particularly during invasive procedures. Chronic urea overload significantly increases cyanate concentrations in patients with ESKD, leading to carbamylation, an irreversible modification of proteins and peptides.

Methods

To investigate carbamylation as a potential mechanistic link between uremia and platelet dysfunction in ESKD, we used liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) to quantify total homocitrulline, and biotin-conjugated phenylglyoxal labeling and Western blot to detect carbamylated integrin α _IIb β ₃ (a receptor required for platelet aggregation). Flow cytometry was used to study activation of isolated platelet

SUBMITTER: Binder V 

PROVIDER: S-EPMC9528322 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature