Netrin G1 Is a Novel Target Antigen in Primary Membranous Nephropathy.

ABSTRACT:

Background

Primary membranous nephropathy (MN) is caused by circulating autoantibodies binding to antigens on the podocyte surface. PLA₂R1 is the main target antigen in 70%-80% of cases, but the pathogenesis is unresolved in 10%-15% of patients.

Methods

We used native western blotting to identify IgG4 autoantibodies, which bind an antigen endogenously expressed on podocyte membranes, in the serum of the index patient with MN. These IgG4 autoantibodies were used to immunoprecipitate the target antigen, and mass spectrometry was used to identify Netrin G1 (NTNG1). Using native western blot and ELISA, NTNG1 autoantibodies were analyzed in cohorts of 888 patients with MN or other glomerular diseases.

Results

NTNG1 was identified as a novel target antigen in MN.

SUBMITTER: Reinhard L 

PROVIDER: S-EPMC9528326 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature