Peripubertal requirement of Tsg101 in maintaining the integrity of membranous structures in mouse oocytes.

ABSTRACT:

Objective

As a component of Endosomal Sorting Complex Required for Transport (ESCRT) complex I, the tumor susceptibility gene 101 (Tsg101) carries out multiple functions. In this work, we report that oocyte-specific deletion of tumor susceptibility gene 101 (Tsg101) leads to age-dependent oocyte demise in mice.

Materials and method

Tsg101 floxed mice (Tsg101^f/f ) were bred with Zp3^cre transgenic mice to examine oocyte-specific roles of Tsg101. Multiple cellular and molecular biological approaches were taken to examine what leads to oocyte demise in the absence of Tsg101.

Results

The death of oocytes from Zp3^cre /Tsg101^f/f (Tsg101^d/d thereafter) mice showed a strong correlation with sexual maturation, as gonadotrop

SUBMITTER: Shin H 

PROVIDER: S-EPMC9528763 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature