Listeria monocytogenes genes supporting growth under standard laboratory cultivation conditions and during macrophage infection.

ABSTRACT: The Gram-positive bacterium Listeria monocytogenes occurs widespread in the environment and infects humans when ingested along with contaminated food. Such infections are particularly dangerous for risk group patients, for whom they represent a life-threatening disease. To invent novel strategies to control contamination and disease, it is important to identify those cellular processes that maintain pathogen growth inside and outside the host. Here, we have applied transposon insertion sequencing (Tn-Seq) to L. monocytogenes for the identification of such processes on a genome-wide scale. Our approach identified 394 open reading frames that are required for growth under standard laboratory conditions and 42 further genes, which become necessary during intracellular growth in

SUBMITTER: Fischer MA 

PROVIDER: S-EPMC9528990 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature