Project description:This study investigated the impact of TRPM8 activity on human monocyte differentiation and function. CD14+ peripheral blood monocytes were isolated from healthy volunteers and differentiated into macrophages using M-CSF, in the presence or absence of 10μM AMTB (TRPM8 antagonist). RNA seq was performed on CD14+ monocytes and macrophages differentiated in vehicle(Mo-M-DMSO) or AMTB (Mo-M-AMTB). To assess the transcriptional changes that normally occur in monocyte to macrophage differentiaiton we compared the Mo-M-DMSO and CD14+ monocyte samples. 3109 genes were significantly down-regulated and 3403 up-regulated in Mo-M-DMSO samples compared to CD14+ monocytes. To investigate the impact of AMTB upon macrophage differentiation we compared the Mo-M-DMSO and Mo-M-AMTB. 556 genes were significantly lower and 421 genes were significantly higher in Mo-M-AMTB samples compared to Mo-M-DMSO samples. 331 out of the 556 genes lower in Mo-M-AMTB samples were genes that were normally up-regulated during differentiaiton and 253 out of the 421 genes higher in the Mo-M-AMTB samples were genes that were normally down-regulated during differentiation indicating that AMTB predominantly acts to inhibit aspects of the differentiation programme.

2022-05-16 | GSE131415 | GEO

Apolipoprotein L1 (APOL1) renal risk variant-mediated podocyte cytotoxicity depends on African haplotype and surface expression.

Project description: Not available

| S-EPMC10866943 | biostudies-literature

Extracellular calcium controls background current and neuronal excitability via an UNC79-UNC80-NALCN cation channel complex.

Project description: Not available

| S-EPMC2987630 | biostudies-literature

Photocatalytic Activity of Ionic Carbon Nitrides is Governed by Cation-Modulated Dark Exciton Dynamics.

Project description: Not available

| S-EPMC12622463 | biostudies-literature

Putative chanzyme activity of TRPM2 cation channel is unrelated to pore gating.

Project description: Not available