RNA-binding protein Elavl1/HuR is required for maintenance of cranial neural crest specification.

ABSTRACT: While neural crest development is known to be transcriptionally controlled via sequential activation of gene regulatory networks (GRNs), recent evidence increasingly implicates a role for post-transcriptional regulation in modulating the output of these regulatory circuits. Using available single-cell RNA-sequencing datasets from avian embryos to identify potential post-transcriptional regulators, we found that Elavl1, which encodes for an RNA-binding protein with roles in transcript stability, was enriched in the premigratory cranial neural crest. Perturbation of Elavl1 resulted in premature neural crest delamination from the neural tube as well as significant reduction in transcripts associated with the neural crest specification GRN, phenotypes that are also observed with downreg

SUBMITTER: Hutchins EJ 

PROVIDER: S-EPMC9529247 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature