The optimal ¹⁸F-fluoromisonidazole PET threshold to define tumor hypoxia in preclinical squamous cell carcinomas using pO₂ electron paramagnetic resonance imaging as reference truth.

ABSTRACT:

Purpose

To identify the optimal threshold in ¹⁸F-fluoromisonidazole (FMISO) PET images to accurately locate tumor hypoxia by using electron paramagnetic resonance imaging (pO₂ EPRI) as ground truth for hypoxia, defined by pO₂ [Formula: see text] 10 mmHg.

Methods

Tumor hypoxia images in mouse models of SCCVII squamous cell carcinoma (n = 16) were acquired in a hybrid PET/EPRI imaging system 2 h post-injection of FMISO. T2-weighted MRI was used to delineate tumor and muscle tissue. Dynamic contrast enhanced (DCE) MRI parametric images of K^trans and v_e were generated to model tumor vascular properties. Images from PET/EPR/MRI were co-registered and resampled to isotropic 0.5 mm voxel resolution for analysis. PET images were

SUBMITTER: Gertsenshteyn I 

PROVIDER: S-EPMC9529789 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature