Evaluation of reference genes for transcript normalization in Fragaria chiloensis fruit and vegetative tissues.

ABSTRACT: Quantitative real-time PCR (RT-qPCR) is used extensively in gene expression studies. For adequate comparisons, the identification and use of reliable reference genes are crucial. Nevertheless, the availability of such genes in strawberry species is limited and has yet to be described for the Chilean strawberry, Fragaria chiloensis. In this study, the expression dynamics of a set of 10 candidate reference genes were analyzed in various F. chiloensis vegetative tissues (root, runners, stem, leaf, and flower), and fruits at different ripening stages or subjected to different hormonal treatments (ABA, auxin). The expression stability of candidate genes was examined by a series of algorithms, such as geNorm, NormFinder, BestKeeper, and ΔCt, for comparisons and rankings. Finally, b

SUBMITTER: Gaete-Eastman C 

PROVIDER: S-EPMC9530087 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature