Distinct role of mitochondrial function and protein kinase C in intimal and medial calcification in vitro.

ABSTRACT:

Introduction

Vascular calcification (VC) is a major risk factor for cardiovascular morbidity and mortality. Depending on the location of mineral deposition within the arterial wall, VC is classified as intimal and medial calcification. Using in vitro mineralization assays, we developed protocols triggering both types of calcification in vascular smooth muscle cells (SMCs) following diverging molecular pathways.

Materials and methods and results

Human coronary artery SMCs were cultured in osteogenic medium (OM) or high calcium phosphate medium (CaP) to induce a mineralized extracellular matrix. OM induces osteoblast-like differentiation of SMCs-a key process in intimal calcification during atherosclerotic plaque remodeling. CaP mimics hyperphosphatemia, associated with

SUBMITTER: Heuschkel MA 

PROVIDER: S-EPMC9530266 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature