Intramammary infusion of matrine-chitosan hydrogels for treating subclinical bovine mastitis -effects on milk microbiome and metabolites.

ABSTRACT:

Background

Bovine metabolism undergoes significant changes during subclinical mastitis, but the relevant molecular mechanisms have not been elucidated. In this study we investigated the changes in milk microbiota and metabolites after intramammary infusion of matrine-chitosan hydrogels (MCHs) in cows with subclinical mastitis.

Methods

Infusions were continued for 7 days, and milk samples were collected on days 1 and 7 for microbiome analysis by 16S rRNA gene sequencing and metabolite profiling by liquid chromatography-mass spectrometry.

Results

MCHs significantly decreased the somatic cell count on day 7 compared to day 1, and the Simpson index indicated that microbial diversity was significantly lower on day 7. The relative abundance of Aerococcus, Coryneba

SUBMITTER: Zhang H 

PROVIDER: S-EPMC9530655 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature