SPICE-Met: profiling and imaging energy metabolism at the single-cell level using a fluorescent reporter mouse.

ABSTRACT: The regulation of cellular energy metabolism is central to most physiological and pathophysiological processes. However, most current methods have limited ability to functionally probe metabolic pathways in individual cells. Here, we describe SPICE-Met (Single-cell Profiling and Imaging of Cell Energy Metabolism), a method for profiling energy metabolism in single cells using flow cytometry or imaging. We generated a transgenic mouse expressing PercevalHR, a fluorescent reporter for cellular ATP:ADP ratio. Modulation of PercevalHR fluorescence with metabolic inhibitors was used to infer the dependence of energy metabolism on oxidative phosphorylation and glycolysis in defined cell populations identified by flow cytometry. We applied SPICE-Met to analyze T-cell memory development during vac

SUBMITTER: Russo E 

PROVIDER: S-EPMC9531294 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature