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Protocol for characterizing the inhibition of SARS-CoV-2 infection by a protein of interest in cultured cells.

ABSTRACT: Here, we present a protocol to characterize the antiviral ability of a protein of interest to SARS-CoV-2 infection in cultured cells, using MUC1 as an example. We use SARS-CoV-2 ΔN trVLP system, which utilizes transcription and replication-competent SARS-CoV-2 virus-like particles lacking nucleocapsid gene. We describe the optimized procedure to analyze protein interference of viral attachment and entry into cells, and qRT-PCR-based quantification of viral infection. The protocol can be applied to characterize more antiviral candidates and clarify their functioning stage. For complete details on the use and execution of this protocol, please refer to Lai et al. (2022).

SUBMITTER: Lai X

PROVIDER: S-EPMC9531662 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Protocol for characterizing the inhibition of SARS-CoV-2 infection by a protein of interest in cultured cells.

Lai Xinyuan X Zhuang Hui H Li Tong T Xiang Kuanhui K

STAR protocols 20221004 4

Here, we present a protocol to characterize the antiviral ability of a protein of interest to SARS-CoV-2 infection in cultured cells, using MUC1 as an example. We use SARS-CoV-2 ΔN trVLP system, which utilizes transcription and replication-competent SARS-CoV-2 virus-like particles lacking nucleocapsid gene. We describe the optimized procedure to analyze protein interference of viral attachment and entry into cells, and qRT-PCR-based quantification of viral infection. The protocol can be applied ...[more]

PMID: 36345374

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