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AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing's sarcoma cells.

ABSTRACT: Ewing's sarcoma is the second most common bone malignancy in children or young adults and is caused by an oncogenic transcription factor by a chromosomal translocation between the EWSR1 gene and the ETS transcription factor family. However, the transcriptional mechanism of EWS-ETS fusion proteins is still unclear. To identify the transcriptional complexes of EWS-ETS fusion transcription factors, we applied a proximal labeling system called BioID in Ewing's sarcoma cells. We identified AHDC1 as a proximal protein of EWS-ETS fusion proteins. AHDC1 knockdown showed a reduced cell growth and transcriptional activity of EWS-FLI1. AHDC1 knockdown also reduced BRD4 and BRG1 protein levels, both known as interacting proteins of EWS-FLI1. Our results suggest that AHDC1 supports cell growth through EWS-FLI1.

SUBMITTER: Kitagawa T 

PROVIDER: S-EPMC9531837 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing's sarcoma cells.

Kitagawa Takao T   Kobayashi Daiki D   Baron Byron B   Okita Hajime H   Miyamoto Tatsuo T   Takai Rie R   Paudel Durga D   Ohta Tohru T   Asaoka Yoichi Y   Tokunaga Masayuki M   Nakagawa Koji K   Furutani-Seiki Makoto M   Araki Norie N   Kuramitsu Yasuhiro Y   Kobayashi Masanobu M  

PloS one 20221004 10

Ewing's sarcoma is the second most common bone malignancy in children or young adults and is caused by an oncogenic transcription factor by a chromosomal translocation between the EWSR1 gene and the ETS transcription factor family. However, the transcriptional mechanism of EWS-ETS fusion proteins is still unclear. To identify the transcriptional complexes of EWS-ETS fusion transcription factors, we applied a proximal labeling system called BioID in Ewing's sarcoma cells. We identified AHDC1 as a  ...[more]

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