Research on safety and compliance of imported microbial inoculants using high-throughput sequencing.

ABSTRACT: Microbial inoculants are widely used in wastewater treatment, soil remediation, and biological control. Safety and compliance for active constituents are considered to be the most important measures of imported microbial inoculants. Microbial inoculants composition was commonly identified by phenotypic culture, which is time-consuming and labor intense with occasionally false negative results provided, and can only be tested for specific species. High-throughput sequencing (HTS), known for its non-targeted detection of unknown species composition in samples, is suitable for composition consistency identification and biosafety analysis of imported microbial inoculants. In this study, the application of HTS for microflora distribution and resistance gene was verified in microbial inoculants

SUBMITTER: Dong L 

PROVIDER: S-EPMC9532531 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature