Endocytosis at extremes: Formation and internalization of giant clathrin-coated pits under elevated membrane tension.

ABSTRACT: Internalization of clathrin-coated vesicles from the plasma membrane constitutes the major endocytic route for receptors and their ligands. Dynamic and structural properties of endocytic clathrin coats are regulated by the mechanical properties of the plasma membrane. Here, we used conventional fluorescence imaging and multiple modes of structured illumination microscopy (SIM) to image formation of endocytic clathrin coats within live cells and tissues of developing fruit fly embryos. High resolution in both spatial and temporal domains allowed us to detect and characterize distinct classes of clathrin-coated structures. Aside from the clathrin pits and plaques detected in distinct embryonic tissues, we report, for the first time, formation of giant coated pits (GCPs) that can be up to two

SUBMITTER: Akatay AA 

PROVIDER: S-EPMC9532848 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature