New plasmid system to select for Saccharomyces cerevisiae purine-cytosine permease affinity mutants.

ABSTRACT: The FCY2 gene of Saccharomyces cerevisiae encodes a purine-cytosine permease (PCP) that mediates the active transport of purines and cytosine. A structure-function model for this PCP has been recently proposed. In this study, we developed a plasmid-based system that generated a number of affinity-mutated alleles, enabling us to define new amino acids critical for permease function.

SUBMITTER: Wagner R 

PROVIDER: S-EPMC95330 | biostudies-literature | 2001 Jul

REPOSITORIES: biostudies-literature