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A multicomponent reaction platform towards multimodal near-infrared BODIPY dyes for STED and fluorescence lifetime imaging.

ABSTRACT: We report a platform combining multicomponent reaction synthesis and automated cell-based screening to develop biocompatible NIR-BODIPY fluorophores. From a library of over 60 fluorophores, we optimised compound NIRBD-62c as a multimodal probe with suitable properties for STED super-resolution and fluorescence lifetime imaging. Furthermore, we employed NIRBD-62c for imaging trafficking inside cells and to examine how pharmacological inhibitors can alter the vesicular traffic between intracellular compartments and the plasma membrane.

SUBMITTER: Fernandez A

PROVIDER: S-EPMC9533399 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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A multicomponent reaction platform towards multimodal near-infrared BODIPY dyes for STED and fluorescence lifetime imaging.

Fernandez Antonio A Kielland Nicola N Makda Ashraff A Carragher Neil O NO González-García M Carmen MC Espinar-Barranco Laura L González-Vera Juan A JA Orte Angel A Lavilla Rodolfo R Vendrell Marc M

RSC chemical biology 20220825 10

We report a platform combining multicomponent reaction synthesis and automated cell-based screening to develop biocompatible NIR-BODIPY fluorophores. From a library of over 60 fluorophores, we optimised compound NIRBD-62c as a multimodal probe with suitable properties for STED super-resolution and fluorescence lifetime imaging. Furthermore, we employed NIRBD-62c for imaging trafficking inside cells and to examine how pharmacological inhibitors can alter the vesicular traffic between intracellula ...[more]

PMID: 36320886

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