LPAR5 confers radioresistance to cancer cells associated with EMT activation via the ERK/Snail pathway.

ABSTRACT:

Background

Epithelial-to-mesenchymal transition (EMT) is a critical event contributing to more aggressive phenotypes in cancer cells. EMT is frequently activated in radiation-targeted cells during the course of radiotherapy, which often endows cancers with acquired radioresistance. However, the upstream molecules driving the signaling pathways of radiation-induced EMT have not been fully delineated.

Methods

In this study, RNA-seq-based transcriptome analysis was performed to identify the early responsive genes of HeLa cells to γ-ray irradiation. EMT-associated genes were knocked down by siRNA technology or overexpressed in HeLa cells and A549 cells, and the resulting changes in phenotypes of EMT and radiosensitivity were assessed using qPCR and Western blotting analyses, mig

SUBMITTER: Sun XY 

PROVIDER: S-EPMC9533496 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature