Development and validation of a CRISPR interference system for gene regulation in Campylobacter jejuni.

ABSTRACT:

Background

Campylobacter spp. are the leading cause of bacterial food-borne illness in humans worldwide, with Campylobacter jejuni responsible for 80% of these infections. There is an urgent need to understand fundamental C. jejuni biology for the development of new strategies to prevent and treat infections. The range of molecular tools available to regulate gene expression in C. jejuni is limited, which in turn constrains our ability to interrogate the function of essential and conditionally essential genes. We have addressed this by developing and utilising a CRISPR-based interference system known as CRISPRi in C. jejuni to control gene expression. To achieve this, a catalytically inactive ("dead") cas9 and sgRNA backbone from the Streptococcus pyogenes CRISPRi system was combin

SUBMITTER: Costigan R 

PROVIDER: S-EPMC9533551 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature