High-throughput detection of T-DNA insertion sites for multiple transgenes in complex genomes.

ABSTRACT:

Background

Genetic engineering of crop plants has been successful in transferring traits into elite lines beyond what can be achieved with breeding techniques. Introduction of transgenes originating from other species has conferred resistance to biotic and abiotic stresses, increased efficiency, and modified developmental programs. The next challenge is now to combine multiple transgenes into elite varieties via gene stacking to combine traits. Generating stable homozygous lines with multiple transgenes requires selection of segregating generations which is time consuming and labor intensive, especially if the crop is polyploid. Insertion site effects and transgene copy number are important metrics for commercialization and trait efficiency.

Results

We have developed a simpl

SUBMITTER: Edwards B 

PROVIDER: S-EPMC9533571 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature