Lipopolysaccharide downregulates the expression of ZO-1 protein through the Akt pathway.

ABSTRACT:

Background

Neonatal bacterial meningitis is a common neonatal disease with high morbidity, and can cause serious sequelae when left untreated. Escherichia coli is the common pathogen, and its endotoxin, lipopolysaccharide (LPS) can damage the endothelial cells, increasing the permeability of the blood-brain barrier (BBB), leading to intracranial inflammation. However, the specific mechanism of bacterial meningitis induced by LPS damaging BBB remains unclear. In this study, the mouse brain microvascular endothelial (bEND.3) cells were used as a research object to investigate whether LPS damage BBB through the PI3K/Akt pathway.

Methods

The bEND.3 cells were stimulated with different concentrations of LPS for 12 h, and the expression of tight junction proteins (ZO-1, claudin-5,

SUBMITTER: Zou P 

PROVIDER: S-EPMC9533599 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature