Adjuvant effect of IRES-based single-stranded RNA on melanoma immunotherapy.

ABSTRACT:

Background

Adjuvant therapies such as radiation therapy, chemotherapy, and immunotherapy are usually given after cancer surgery to improve the survival of cancer patients. However, despite advances in several adjuvant therapies, they are still limited in the prevention of recurrences.

Methods

We evaluated the immunological effects of RNA-based adjuvants in a murine melanoma model. Single-stranded RNA (ssRNA) were constructed based on the cricket paralysis virus (CrPV) internal ribosome entry site (IRES). Populations of immune cells in bone marrow cells and lymph node cells following immunization with CrPV^IRES-ssRNA were determined using flow cytometry. Activated cytokine levels were measured using ELISA and ELISpot. The tumor protection efficacy of CrPV^IRES</

SUBMITTER: Kwak HW 

PROVIDER: S-EPMC9533600 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature