Dataset Information

Real-time PCR assay for the detection of tanapox virus and yaba-like disease virus.

ABSTRACT: The yatapoxvirus genus contains three members: tanapox virus (TPV), yaba-like disease virus (YLDV) and yaba monkey tumor virus (YMTV), two of which (TPV and YLDV) may infect humans. However, only a very small number of patients have been diagnosed with TPV outside Africa. Given the increased international travel and the similarity of clinical signs during the early stages of a TPV/YLDV infection as compared to diseases caused by agents of potential biological warfare, such as smallpox, monkeypox, tularemia and anthrax, the rapid and reliable recognition of a TPV/YLDV infection is crucial. A real-time PCR assay using TaqManchemistry was developed in order to identify unambiguously TPV/YLDV. Primers and probe targeting a 101bp region of the PstI L fragment of TPV, initial optimisations steps were carried out with YLDV DNA as template. Using probit regression analysis, the lower limit of detection was calculated to be ca. 8 copies per assay. A total of five TPV strains, one YDLV strain and scab-derived DNA from a patient with a TPV infection yielded specific amplification, whereas the DNA of YMTV was not amplified. Various viral and bacterial pathogens (n=29) associated with rash-causing illnesses were not detected using this assay.

SUBMITTER: Zimmermann P

PROVIDER: S-EPMC9533964 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature

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Real-time PCR assay for the detection of tanapox virus and yaba-like disease virus.

Zimmermann Pia P Thordsen Ingo I Frangoulidis Dimitrios D Meyer Hermann H

Journal of virological methods 20050728 1-2

The yatapoxvirus genus contains three members: tanapox virus (TPV), yaba-like disease virus (YLDV) and yaba monkey tumor virus (YMTV), two of which (TPV and YLDV) may infect humans. However, only a very small number of patients have been diagnosed with TPV outside Africa. Given the increased international travel and the similarity of clinical signs during the early stages of a TPV/YLDV infection as compared to diseases caused by agents of potential biological warfare, such as smallpox, monkeypox ...[more]

PMID: 16054706

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Dataset Information

Real-time PCR assay for the detection of tanapox virus and yaba-like disease virus.

Publications

Real-time PCR assay for the detection of tanapox virus and yaba-like disease virus.

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