Tracing the oomycete pathogen Saprolegnia parasitica in aquaculture and the environment.

ABSTRACT: Saprolegnia parasitica causes saprolegniosis, a disease responsible for significant economic losses in aquaculture and declines of fish populations in the wild, but the knowledge of its distribution and prevalence in the environment is limited. We developed a fast, sensitive and specific S. parasitica droplet digital PCR (ddPCR) assay and demonstrated its applicability for the detection and quantification of the pathogen in environmental samples: swab DNA collected from the host (trout skin, surface of eggs) and environmental DNA extracted from water. The developed assay was used to assess how abiotic (i.e. physico-chemical parameters of the water) and biotic (health status of the host) factors influence the S. parasitica load in the environment. The pathogen load in water samples was posi

SUBMITTER: Pavic D 

PROVIDER: S-EPMC9534867 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature