Determination of Sialic Acid Isomers from Released N-Glycans Using Ion Mobility Spectrometry.

ABSTRACT: Complex carbohydrates are ubiquitous in nature and represent one of the major classes of biopolymers. They can exhibit highly diverse structures with multiple branched sites as well as a complex regio- and stereochemistry. A common way to analytically address this complexity is liquid chromatography (LC) in combination with mass spectrometry (MS). However, MS-based detection often does not provide sufficient information to distinguish glycan isomers. Ion mobility-mass spectrometry (IM-MS)─a technique that separates ions based on their size, charge, and shape─has recently shown great potential to solve this problem by identifying characteristic isomeric glycan features such as the sialylation and fucosylation pattern. However, while both LC-MS and IM-MS have clearly proven their individual

SUBMITTER: Manz C 

PROVIDER: S-EPMC9535620 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature