Peptide Mass Spectra from Micrometer-Thick Ice Films Produced with Femtosecond Pulses.

ABSTRACT: We present a cryogenic mass spectrometry protocol with the capability to detect peptides in the attomole dilution range from ice films. Our approach employs femtosecond laser pulses and implements neither substrate modification nor proton donor agents in the aqueous solution, known to facilitate analyte detection in mass spectrometry. In a systematic study, we investigated the impact of temperature, substrate composition, and irradiation wavelength (513 and 1026 nm) on the bradykinin signal onset. Our findings show that substrate choice and irradiation wavelength have a minor impact on signal intensity once the preparation protocol is optimized. However, if the temperature is increased from -140 to 0 °C, which is accompanied by ice film thinning, a somehow complex picture of analyte desorp

SUBMITTER: Krutilin A 

PROVIDER: S-EPMC9535622 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature