Augmentation of the Photoreactivation Gene in Fremyella diplosiphon Confers UV-B Tolerance.

ABSTRACT: In spite of the enormous potential of cyanobacteria as a renewable energy source, elevated UV exposure is a major impediment to their commercial viability and productivity. Fremyella diplosiphon is a widely explored cyanobacterium with great biofuel capacity due to its high lipid content. To enhance UV stress tolerance in this species, we overexpressed the photoreactivation gene (phr A) that encodes for photolyase DNA repair enzyme in the wild type F. diplosiphon (B481-WT) by genetic transformation. Our efforts resulted in a transformant (B481-ViAnSa) with a 3808-fold increase in the phr A mRNA transcript level and enhanced growth under UV-B stress. Additionally, DNA strand breaks in the transformant were significantly lower after 12 and 16 h of UV radiation, wi

SUBMITTER: Gichuki SM 

PROVIDER: S-EPMC9535648 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature