Jag2b-Notch3/1b-mediated neuron-to-glia crosstalk controls retinal gliogenesis.

ABSTRACT: In the developing central nervous systems (CNS), neural progenitor cells generate neurons and glia in sequential order. However, the influence of neurons on glia generation remains elusive. Here, we report that photoreceptor cell-derived Jag2b is required for Notch-dependent Müller glia (MG) generation in the developing zebrafish retina. In jab2b^-/- mutants, differentiating MGs are re-specified into lineage-related bipolar neuron fate at the expense of mature MG. Single-cell transcriptome analysis and knock-in animals reveal that jab2b is specifically expressed in crx⁺ -photoreceptor cells during MG generation. Crx promoter-driven jag2b, but not other Notch ligands, is sufficient to rescue the loss of MGs observed in jag2b^-/- mutants. Furthermore, we observ

SUBMITTER: Jin M 

PROVIDER: S-EPMC9535768 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature