Constricted migration is associated with stable 3D genome structure differences in cancer cells.

ABSTRACT: To spread from a localized tumor, metastatic cancer cells must squeeze through constrictions that cause major nuclear deformations. Since chromosome structure affects nucleus stiffness, gene regulation, and DNA repair, here, we investigate the relationship between 3D genome structure and constricted migration in cancer cells. Using melanoma (A375) cells, we identify phenotypic differences in cells that have undergone multiple rounds of constricted migration. These cells display a stably higher migration efficiency, elongated morphology, and differences in the distribution of Lamin A/C and heterochromatin. Hi-C experiments reveal differences in chromosome spatial compartmentalization specific to cells that have passed through constrictions and related alterations in expression of genes asso

SUBMITTER: Golloshi R 

PROVIDER: S-EPMC9535800 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature