DNA-assisted selective electrofusion (DASE) of Escherichia coli and giant lipid vesicles.

ABSTRACT: Synthetic biology and cellular engineering require chemical and physical alterations, which are typically achieved by fusing target cells with each other or with payload-carrying vectors. On one hand, electrofusion can efficiently induce the merging of biological cells and/or synthetic analogues via the application of intense DC pulses, but it lacks selectivity and often leads to uncontrolled fusion. On the other hand, synthetic DNA-based constructs, inspired by natural fusogenic proteins, have been shown to induce a selective fusion between membranes, albeit with low efficiency. Here we introduce DNA-assisted selective electrofusion (DASE) which relies on membrane-anchored DNA constructs to bring together the objects one seeks to merge, and applying an electric impulse to trigger t

SUBMITTER: Takamori S 

PROVIDER: S-EPMC9536516 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature