Prostaglandins and calprotectin are genetically and functionally linked to the Inflammatory Bowel Diseases.

ABSTRACT:

Background

Genome wide association studies (GWAS) have identified and validated more than 200 genomic loci associated with the inflammatory bowel disease (IBD), although for most the causal gene remains unknown. Given the importance of myeloid cells in IBD pathogenesis, the current study aimed to uncover the role of genes within IBD genetic loci that are endogenously expressed in this cell lineage.

Methods

The open reading frames (ORF) of 42 genes from IBD-associated loci were expressed via lentiviral transfer in the THP-1 model of human monocytes and the impact of each of these on the cell's transcriptome was analyzed using a RNA sequencing-based approach. We used a combination of genetic and pharmacologic approaches to validate our findings in the THP-1 line with further v

SUBMITTER: Karaky M 

PROVIDER: S-EPMC9536535 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature