Using Fluorescence Recovery After Photobleaching data to uncover filament dynamics.

ABSTRACT: Fluorescence Recovery After Photobleaching (FRAP) has been extensively used to understand molecular dynamics in cells. This technique when applied to soluble, globular molecules driven by diffusion is easily interpreted and well understood. However, the classical methods of analysis cannot be applied to anisotropic structures subjected to directed transport, such as cytoskeletal filaments or elongated organelles transported along microtubule tracks. A new mathematical approach is needed to analyze FRAP data in this context and determine what information can be obtain from such experiments. To address these questions, we analyze fluorescence intensity profile curves after photobleaching of fluorescently labelled intermediate filaments anterogradely transported along microtubules. We apply t

SUBMITTER: Dallon JC 

PROVIDER: S-EPMC9536589 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature